Overview:
Embark on a journey into the world of mycology with our premium Lion’s Mane Mushroom Liquid Culture Syringe. Designed for the mycology enthusiast, this tool is your gateway to cultivating the extraordinary Lion’s Mane mushrooms, renowned for their unique neurotropic benefits and culinary versatility.

Optimized for Success, each syringe is meticulously prepared with a high-potency liquid culture, ensuring a rapid and healthy mycelium expansion. Ideal for intermediate enthusiasts, it offers a straightforward application with rewarding outcomes.

Key Features:
•10ml of premium, high-density Lion’s Mane liquid culture
•Sterilized, ready-to-use syringe with needle tip included
•Long shelf life with storage guidelines provided

Enhance Your Culinary & Health Journey:
Lion’s Mane mushrooms are not only a gourmet delight but also a superfood for your brain. Cultivating your own ensures you have the freshest, most potent mushrooms at your fingertips, perfect for culinary experiments or nutritional supplements.

Your liquid culture syringe is prepared under strict sterile conditions to prevent contamination and ensure the highest viability of the culture. It is then sealed in a sterile, airtight zip-lock package, preserving its purity and readiness for your cultivation projects. Our package will be in a padded shipping envelope. This ensures that the syringe and its contents are shielded from physical damage, maintaining quality of the culture until it reaches your hands.

Storing the Lion’s Mane Mushroom Liquid Culture Syringe Before Use

Keep It Refrigerated:
Upon receiving your liquid culture syringe, place it in the refrigerator if you do not plan to use it immediately. The ideal storage temperature is between 35°F to 46°F (2°C to 8°C). This cool environment helps maintain the culture's viability without putting it into a dormant state.

Avoid Freezing:
Do not freeze the liquid culture syringe. Freezing can damage the mycelium cells, significantly reducing their viability and potentially rendering the culture useless for inoculation purposes.

Storing Unused Portions of the Liquid Culture Syringe After Use

Re-sterilize the Needle:
After the initial use, if you have remaining culture in the syringe, flame sterilize the needle again by passing it through a flame until it glows red, then allow it to cool. This step is crucial to prevent introducing contaminants into the syringe when it is used next.

Cap Securely:
Replace the needle cap securely to prevent any contamination and leakage.

Refrigerate Again:
Place the syringe in a Ziploc bag and return the syringe to the refrigerator for storage, following the same guidelines as before use. This will help preserve the remaining culture until you're ready to use it again.

Label with Date:
It's helpful to label the syringe with the date of first use. Most liquid cultures can be stored for several months in the refrigerator, but it's good practice to use them within the shortest possible time frame to ensure the best results.

Check for Contamination:
Before reusing the syringe, check for any signs of contamination. If the liquid appears extra thick and cloudy or has unusual colors, it may be contaminated and should not be used.

Prepare Your Workspace:
Ensure your workspace is clean and as free from drafts and contaminants as possible. Wiping down work surfaces, grain bags, and syringes with isopropyl alcohol and wearing gloves can help maintain a sterile environment.

Grain:
Sterilized grain, such as corn or rye berry grain, is required and can be purchased from numerous vendors online or prepared at home using a pressure cooker.

Shake the Syringe: Before use, gently shake the syringe to evenly distribute the mycelium throughout the liquid culture. This helps ensure an even inoculation of the grain.

Flame Sterilize the Needle:
Using a lighter or a small torch, carefully heat the metal needle tip of the syringe until it is red hot. This sterilizes the needle, eliminating any potential contaminants. Allow the needle to cool for a few seconds before proceeding.

Inoculate the Grain:
Pierce the sterile needle through the sterilized grain bag's injection port or through a small hole in the lid of your sterilized grain jar (which should then be covered with micropore tape or a similar breathable filter). Inject a small amount of the liquid culture into the grain. The exact amount can vary dependent upon the amount of grain being used, but a general guideline is 1-2 ml of culture per quart of grain.

Incubate:
Place the inoculated grain in a dark, warm area with a temperature around 66°-75°F (19°-24°C) to incubate. Avoid direct sunlight and excessive heat or cold, which can hinder mycelium growth.

Wait and Observe:
Over the next few weeks, you should observe white mycelial growth spreading throughout the grain. This indicates successful inoculation and colonization. Once the grain is fully colonized, it's ready for the next step in your mushroom cultivation process, usually transferring to a fruiting substrate.